polyclonal rabbit kappa-fitc Search Results


polyclonal goat anti mouse igm  (SouthernBiotech)
91
SouthernBiotech polyclonal goat anti mouse igm
Polyclonal Goat Anti Mouse Igm, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal nf kb p65  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology rabbit polyclonal nf kb p65
Figure 4. Mw induces <t>NF-kB–p65</t> activation in infected macrophages. Uninfected macrophages (UIM) or Leishmania-infected macrophages (IM) were treated as described in the legend of Figure 1 for 0, 15, 30, 60 and 120 min at 378C. Cytosolic and nuclear protein extracts were prepared. Subsequently, western blot analysis was performed with anti-p65 to study the distribution of NF-kB–p65 in the cytoplasm (Cyto) and nucleus (Nuc) at different timepoints; equal protein loading was evaluated by GAPDH (a and b). Data represent the mean+SD of at least three independent experiments. In another experimental set, uninfected or L. donovani-infected macrophages were treated as above for 1 h, followed by electrophoretic mobility shift assay using differently treated nuclear extracts with labelled NF-kB–p65 probe to analyse the nuclear translocation and DNA binding of NF-kB–p65 in uninfected and L. donovani-infected macrophages; non-labelled (‘cold’) oligonucleotide NF-kB probe was used as a cold probe and normal macrophages stimulated with LPS (1 mg/mL) served as a positive control (c). The autoradiographs are representative of three independent experiments that had identical results.
Rabbit Polyclonal Nf Kb P65, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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kappa fitc bd rage 1°ab igg2a  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology kappa fitc bd rage 1°ab igg2a
Figure 4. Mw induces <t>NF-kB–p65</t> activation in infected macrophages. Uninfected macrophages (UIM) or Leishmania-infected macrophages (IM) were treated as described in the legend of Figure 1 for 0, 15, 30, 60 and 120 min at 378C. Cytosolic and nuclear protein extracts were prepared. Subsequently, western blot analysis was performed with anti-p65 to study the distribution of NF-kB–p65 in the cytoplasm (Cyto) and nucleus (Nuc) at different timepoints; equal protein loading was evaluated by GAPDH (a and b). Data represent the mean+SD of at least three independent experiments. In another experimental set, uninfected or L. donovani-infected macrophages were treated as above for 1 h, followed by electrophoretic mobility shift assay using differently treated nuclear extracts with labelled NF-kB–p65 probe to analyse the nuclear translocation and DNA binding of NF-kB–p65 in uninfected and L. donovani-infected macrophages; non-labelled (‘cold’) oligonucleotide NF-kB probe was used as a cold probe and normal macrophages stimulated with LPS (1 mg/mL) served as a positive control (c). The autoradiographs are representative of three independent experiments that had identical results.
Kappa Fitc Bd Rage 1°Ab Igg2a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human kappa chain  (SouthernBiotech)
93
SouthernBiotech anti human kappa chain
Figure 4. Mw induces <t>NF-kB–p65</t> activation in infected macrophages. Uninfected macrophages (UIM) or Leishmania-infected macrophages (IM) were treated as described in the legend of Figure 1 for 0, 15, 30, 60 and 120 min at 378C. Cytosolic and nuclear protein extracts were prepared. Subsequently, western blot analysis was performed with anti-p65 to study the distribution of NF-kB–p65 in the cytoplasm (Cyto) and nucleus (Nuc) at different timepoints; equal protein loading was evaluated by GAPDH (a and b). Data represent the mean+SD of at least three independent experiments. In another experimental set, uninfected or L. donovani-infected macrophages were treated as above for 1 h, followed by electrophoretic mobility shift assay using differently treated nuclear extracts with labelled NF-kB–p65 probe to analyse the nuclear translocation and DNA binding of NF-kB–p65 in uninfected and L. donovani-infected macrophages; non-labelled (‘cold’) oligonucleotide NF-kB probe was used as a cold probe and normal macrophages stimulated with LPS (1 mg/mL) served as a positive control (c). The autoradiographs are representative of three independent experiments that had identical results.
Anti Human Kappa Chain, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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s100a8 mac387 igg1 fitc antibody  (GeneTex)
90
GeneTex s100a8 mac387 igg1 fitc antibody
Figure 4. Mw induces <t>NF-kB–p65</t> activation in infected macrophages. Uninfected macrophages (UIM) or Leishmania-infected macrophages (IM) were treated as described in the legend of Figure 1 for 0, 15, 30, 60 and 120 min at 378C. Cytosolic and nuclear protein extracts were prepared. Subsequently, western blot analysis was performed with anti-p65 to study the distribution of NF-kB–p65 in the cytoplasm (Cyto) and nucleus (Nuc) at different timepoints; equal protein loading was evaluated by GAPDH (a and b). Data represent the mean+SD of at least three independent experiments. In another experimental set, uninfected or L. donovani-infected macrophages were treated as above for 1 h, followed by electrophoretic mobility shift assay using differently treated nuclear extracts with labelled NF-kB–p65 probe to analyse the nuclear translocation and DNA binding of NF-kB–p65 in uninfected and L. donovani-infected macrophages; non-labelled (‘cold’) oligonucleotide NF-kB probe was used as a cold probe and normal macrophages stimulated with LPS (1 mg/mL) served as a positive control (c). The autoradiographs are representative of three independent experiments that had identical results.
S100a8 Mac387 Igg1 Fitc Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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s100a9 (1°ab) 4g9 igg1 abnova antibody  (Abnova)
90
Abnova s100a9 (1°ab) 4g9 igg1 abnova antibody
Figure 4. Mw induces <t>NF-kB–p65</t> activation in infected macrophages. Uninfected macrophages (UIM) or Leishmania-infected macrophages (IM) were treated as described in the legend of Figure 1 for 0, 15, 30, 60 and 120 min at 378C. Cytosolic and nuclear protein extracts were prepared. Subsequently, western blot analysis was performed with anti-p65 to study the distribution of NF-kB–p65 in the cytoplasm (Cyto) and nucleus (Nuc) at different timepoints; equal protein loading was evaluated by GAPDH (a and b). Data represent the mean+SD of at least three independent experiments. In another experimental set, uninfected or L. donovani-infected macrophages were treated as above for 1 h, followed by electrophoretic mobility shift assay using differently treated nuclear extracts with labelled NF-kB–p65 probe to analyse the nuclear translocation and DNA binding of NF-kB–p65 in uninfected and L. donovani-infected macrophages; non-labelled (‘cold’) oligonucleotide NF-kB probe was used as a cold probe and normal macrophages stimulated with LPS (1 mg/mL) served as a positive control (c). The autoradiographs are representative of three independent experiments that had identical results.
S100a9 (1°Ab) 4g9 Igg1 Abnova Antibody, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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kappa abcom inos n 20 igg1 fitc santa cruz arginase 1  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology kappa abcom inos n 20 igg1 fitc santa cruz arginase 1
Figure 4. Mw induces <t>NF-kB–p65</t> activation in infected macrophages. Uninfected macrophages (UIM) or Leishmania-infected macrophages (IM) were treated as described in the legend of Figure 1 for 0, 15, 30, 60 and 120 min at 378C. Cytosolic and nuclear protein extracts were prepared. Subsequently, western blot analysis was performed with anti-p65 to study the distribution of NF-kB–p65 in the cytoplasm (Cyto) and nucleus (Nuc) at different timepoints; equal protein loading was evaluated by GAPDH (a and b). Data represent the mean+SD of at least three independent experiments. In another experimental set, uninfected or L. donovani-infected macrophages were treated as above for 1 h, followed by electrophoretic mobility shift assay using differently treated nuclear extracts with labelled NF-kB–p65 probe to analyse the nuclear translocation and DNA binding of NF-kB–p65 in uninfected and L. donovani-infected macrophages; non-labelled (‘cold’) oligonucleotide NF-kB probe was used as a cold probe and normal macrophages stimulated with LPS (1 mg/mL) served as a positive control (c). The autoradiographs are representative of three independent experiments that had identical results.
Kappa Abcom Inos N 20 Igg1 Fitc Santa Cruz Arginase 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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cd79a antibody  (NSJ Bioreagents)
99
NSJ Bioreagents cd79a antibody
Figure 4. Mw induces <t>NF-kB–p65</t> activation in infected macrophages. Uninfected macrophages (UIM) or Leishmania-infected macrophages (IM) were treated as described in the legend of Figure 1 for 0, 15, 30, 60 and 120 min at 378C. Cytosolic and nuclear protein extracts were prepared. Subsequently, western blot analysis was performed with anti-p65 to study the distribution of NF-kB–p65 in the cytoplasm (Cyto) and nucleus (Nuc) at different timepoints; equal protein loading was evaluated by GAPDH (a and b). Data represent the mean+SD of at least three independent experiments. In another experimental set, uninfected or L. donovani-infected macrophages were treated as above for 1 h, followed by electrophoretic mobility shift assay using differently treated nuclear extracts with labelled NF-kB–p65 probe to analyse the nuclear translocation and DNA binding of NF-kB–p65 in uninfected and L. donovani-infected macrophages; non-labelled (‘cold’) oligonucleotide NF-kB probe was used as a cold probe and normal macrophages stimulated with LPS (1 mg/mL) served as a positive control (c). The autoradiographs are representative of three independent experiments that had identical results.
Cd79a Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human c3 c3d fitc  (Valiant Co Ltd)
96
Valiant Co Ltd anti human c3 c3d fitc
Figure 4. Mw induces <t>NF-kB–p65</t> activation in infected macrophages. Uninfected macrophages (UIM) or Leishmania-infected macrophages (IM) were treated as described in the legend of Figure 1 for 0, 15, 30, 60 and 120 min at 378C. Cytosolic and nuclear protein extracts were prepared. Subsequently, western blot analysis was performed with anti-p65 to study the distribution of NF-kB–p65 in the cytoplasm (Cyto) and nucleus (Nuc) at different timepoints; equal protein loading was evaluated by GAPDH (a and b). Data represent the mean+SD of at least three independent experiments. In another experimental set, uninfected or L. donovani-infected macrophages were treated as above for 1 h, followed by electrophoretic mobility shift assay using differently treated nuclear extracts with labelled NF-kB–p65 probe to analyse the nuclear translocation and DNA binding of NF-kB–p65 in uninfected and L. donovani-infected macrophages; non-labelled (‘cold’) oligonucleotide NF-kB probe was used as a cold probe and normal macrophages stimulated with LPS (1 mg/mL) served as a positive control (c). The autoradiographs are representative of three independent experiments that had identical results.
Anti Human C3 C3d Fitc, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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goat anti rabbit secondary  (Jackson Immuno)
96
Jackson Immuno goat anti rabbit secondary
Figure 4. Mw induces <t>NF-kB–p65</t> activation in infected macrophages. Uninfected macrophages (UIM) or Leishmania-infected macrophages (IM) were treated as described in the legend of Figure 1 for 0, 15, 30, 60 and 120 min at 378C. Cytosolic and nuclear protein extracts were prepared. Subsequently, western blot analysis was performed with anti-p65 to study the distribution of NF-kB–p65 in the cytoplasm (Cyto) and nucleus (Nuc) at different timepoints; equal protein loading was evaluated by GAPDH (a and b). Data represent the mean+SD of at least three independent experiments. In another experimental set, uninfected or L. donovani-infected macrophages were treated as above for 1 h, followed by electrophoretic mobility shift assay using differently treated nuclear extracts with labelled NF-kB–p65 probe to analyse the nuclear translocation and DNA binding of NF-kB–p65 in uninfected and L. donovani-infected macrophages; non-labelled (‘cold’) oligonucleotide NF-kB probe was used as a cold probe and normal macrophages stimulated with LPS (1 mg/mL) served as a positive control (c). The autoradiographs are representative of three independent experiments that had identical results.
Goat Anti Rabbit Secondary, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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fluorescein isothiocyanate fitc  (St Johns Laboratory)
90
St Johns Laboratory fluorescein isothiocyanate fitc
Figure 4. Mw induces <t>NF-kB–p65</t> activation in infected macrophages. Uninfected macrophages (UIM) or Leishmania-infected macrophages (IM) were treated as described in the legend of Figure 1 for 0, 15, 30, 60 and 120 min at 378C. Cytosolic and nuclear protein extracts were prepared. Subsequently, western blot analysis was performed with anti-p65 to study the distribution of NF-kB–p65 in the cytoplasm (Cyto) and nucleus (Nuc) at different timepoints; equal protein loading was evaluated by GAPDH (a and b). Data represent the mean+SD of at least three independent experiments. In another experimental set, uninfected or L. donovani-infected macrophages were treated as above for 1 h, followed by electrophoretic mobility shift assay using differently treated nuclear extracts with labelled NF-kB–p65 probe to analyse the nuclear translocation and DNA binding of NF-kB–p65 in uninfected and L. donovani-infected macrophages; non-labelled (‘cold’) oligonucleotide NF-kB probe was used as a cold probe and normal macrophages stimulated with LPS (1 mg/mL) served as a positive control (c). The autoradiographs are representative of three independent experiments that had identical results.
Fluorescein Isothiocyanate Fitc, supplied by St Johns Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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percp  (Agilent technologies)
95
Agilent technologies percp
Figure 4. Mw induces <t>NF-kB–p65</t> activation in infected macrophages. Uninfected macrophages (UIM) or Leishmania-infected macrophages (IM) were treated as described in the legend of Figure 1 for 0, 15, 30, 60 and 120 min at 378C. Cytosolic and nuclear protein extracts were prepared. Subsequently, western blot analysis was performed with anti-p65 to study the distribution of NF-kB–p65 in the cytoplasm (Cyto) and nucleus (Nuc) at different timepoints; equal protein loading was evaluated by GAPDH (a and b). Data represent the mean+SD of at least three independent experiments. In another experimental set, uninfected or L. donovani-infected macrophages were treated as above for 1 h, followed by electrophoretic mobility shift assay using differently treated nuclear extracts with labelled NF-kB–p65 probe to analyse the nuclear translocation and DNA binding of NF-kB–p65 in uninfected and L. donovani-infected macrophages; non-labelled (‘cold’) oligonucleotide NF-kB probe was used as a cold probe and normal macrophages stimulated with LPS (1 mg/mL) served as a positive control (c). The autoradiographs are representative of three independent experiments that had identical results.
Percp, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 4. Mw induces NF-kB–p65 activation in infected macrophages. Uninfected macrophages (UIM) or Leishmania-infected macrophages (IM) were treated as described in the legend of Figure 1 for 0, 15, 30, 60 and 120 min at 378C. Cytosolic and nuclear protein extracts were prepared. Subsequently, western blot analysis was performed with anti-p65 to study the distribution of NF-kB–p65 in the cytoplasm (Cyto) and nucleus (Nuc) at different timepoints; equal protein loading was evaluated by GAPDH (a and b). Data represent the mean+SD of at least three independent experiments. In another experimental set, uninfected or L. donovani-infected macrophages were treated as above for 1 h, followed by electrophoretic mobility shift assay using differently treated nuclear extracts with labelled NF-kB–p65 probe to analyse the nuclear translocation and DNA binding of NF-kB–p65 in uninfected and L. donovani-infected macrophages; non-labelled (‘cold’) oligonucleotide NF-kB probe was used as a cold probe and normal macrophages stimulated with LPS (1 mg/mL) served as a positive control (c). The autoradiographs are representative of three independent experiments that had identical results.

Journal: The Journal of antimicrobial chemotherapy

Article Title: Mycobacterium indicus pranii (Mw)-mediated protection against visceral leishmaniasis: involvement of TLR4 signalling.

doi: 10.1093/jac/dks315

Figure Lengend Snippet: Figure 4. Mw induces NF-kB–p65 activation in infected macrophages. Uninfected macrophages (UIM) or Leishmania-infected macrophages (IM) were treated as described in the legend of Figure 1 for 0, 15, 30, 60 and 120 min at 378C. Cytosolic and nuclear protein extracts were prepared. Subsequently, western blot analysis was performed with anti-p65 to study the distribution of NF-kB–p65 in the cytoplasm (Cyto) and nucleus (Nuc) at different timepoints; equal protein loading was evaluated by GAPDH (a and b). Data represent the mean+SD of at least three independent experiments. In another experimental set, uninfected or L. donovani-infected macrophages were treated as above for 1 h, followed by electrophoretic mobility shift assay using differently treated nuclear extracts with labelled NF-kB–p65 probe to analyse the nuclear translocation and DNA binding of NF-kB–p65 in uninfected and L. donovani-infected macrophages; non-labelled (‘cold’) oligonucleotide NF-kB probe was used as a cold probe and normal macrophages stimulated with LPS (1 mg/mL) served as a positive control (c). The autoradiographs are representative of three independent experiments that had identical results.

Article Snippet: The fixed cells were then incubated with FITC-conjugated anti-TLR4 antibodies (Santa Cruz Biotechnology Inc., USA) and rabbit polyclonal NF-kB–p65 (Santa Cruz Biotechnology Inc., USA) followed by goat anti-rabbit IgG–FITC conjugate (Santa Cruz Biotechnology Inc., USA).

Techniques: Activation Assay, Infection, Western Blot, Electrophoretic Mobility Shift Assay, Translocation Assay, Binding Assay, Positive Control